UV images

UV and phase separation

Here is the image without UV.

UV and phase separation

With the UV, it is much easier to spot the crystals inside the phase separation.

See tutorial 3 for more information about phase separation.

UV and phase separation

 

But what to do about crystals like this? They are too small to put in the beam, but they can be used as seeds. This is exactly what I did. These crystals grew in one of the conditions in the Morpheus screen. I made a seed stock of them, then reseeded all 96 conditions of the Morpheus screen. This is known as matrix microseeding (see the tutorial on seeding for more details.)

After optimization

Here are the crystals that grew 24 hours later. The structure, Pseudomonas aeruginosa IspC, was solved with them.

After optimization

UV image of the above crystals.